Analysis Note

ControlPOSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.

Application

Immunoblotting: 0.5-1 µg/mL

Immunohistochemistry (frozen and formalin/paraffin): 2-4 µg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunoprecpitation: 2 µg/mg protein lysate (use Protein G)

Flow cytometry

Optimal working dilutions must be determined by end user.

This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.

Immunogen

HSP70 from HeLa cells.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Specificity

Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.

POSITIVE CONTROL:

HeLa cells, BT474 cells or breast carcinoma.